Sacsin Chaperone Activity - BIOC 396 Undergraduate Research Project Application Form

Supervisor's Name: Jason Young

Supervisor's Email: jason.young2 [at] mcgill.ca

Supervisor's Phone: 2006

Supervisor's Website:

Supervisor's department: Biochemistry

Course number: BIOC 396 (Biochemistry)

Term: Fall 2015-2016

Project start date: Friday, September 8, 2015

Project end date: Friday, December 4, 2015

Project title: Sacsin Chaperone Activity

Project description (50-100 words suggested): Mutations in the sacsin protein are the cause of Spastic Ataxia of the Charlevoix-Saguenay. Sacsin contains a J domain that activates Hsp70 and a HEPN domain of unknown function next to it. Disease mutations are found in both domains. We hypothesize that sacsin activates the chaperone Hsp70 to bind a specific set of polypeptide substrates, resulting in biological effects of sacsin. The HEPN domain may stabilize the J domain, or regulate it. The activities of wild-type and mutant J-HEPN fragments will be compared as purified proteins. Effects of ligands that bind the HEPN domain will also be examined.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% report, 50% performance

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.