The Measurement of Profile Area of the Cytoplasmic Droplet (CD) of Rat Sperm in the Testis and Epididymis - ANAT 396 Undergraduate Research Project Application Form

Supervisor's Name: Dr. Louis Hermo

Supervisor's Email: louis.hermo [at] mcgill.ca

Supervisor's Phone: 514-398-6348

Supervisor's Website:

Supervisor's department: Anatomy and Cell Biology

Course number: ANAT 396 (Anatomy and Cell Biology)

Term: Fall 2015-2016

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: The Measurement of Profile Area of the Cytoplasmic Droplet (CD) of Rat Sperm in the Testis and Epididymis

Project description (50-100 words suggested): The CD is a small portion of the cytoplasm that is retained along the midpiece of the flagellum of sperm. The focus of this project is to investigate quantitatively the change in size of the Hermes body as sperm are transported from the seminiferous tubule to the epididymis. At the last step of germ cell development, step 19 spermatids, these cells are released into the lumen of the seminiferous tubules to then enter the epididymal duct. As sperm move through the epididymis, they become fertile in the initial segment and then are stored in the cauda.
The measurement of diameter of the CD will be performed by using ImageJ software on immunohistochemical images obtained from staining the CD of three different rats with 3 different antibodies and of sperm at step 19, initial segment and cauda regions. The standard statistical analysis is necessary to show the signficance level of any difference in diameter. We hypothesize that the CD will increase in size from step 19 to the initial segment and then decrease as they reach the cauda region.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% Performance and 50% Lab Report

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

How students can apply / Next steps: Bring a printed copy of this application form and your advising transcript to me during office hours.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.