'Glucose Transporter Expression in SCG' - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Dr. Ellis Cooper

Supervisor's Email: ellis.cooper [at] mcgill.ca

Supervisor's Phone: 514 398 4334

Supervisor's Website:

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Glucose Transporter Expression in SCG

Project description (50-100 words suggested): In mammalian cells, glucose is taken up through facilitative glucose transporters (GLUTs) and metabolized for energy. Excess extracellular glucose is toxic to cells and most individuals suffering from diabetes develop severe complications, particularly in the sympathetic nervous system. Conceivably, glucose uptake could be regulated by targeting GLUTs. However, it is unknown which of the fourteen GLUT members are expressed in sympathetic neurons. Recent results indicate that GLUT1, GLUT3, GLUT6, GLUT8, and GLUT13 are expressed in superior cervical ganglia (SCG) neurons, with GLUT3 being predominantly expressed. This project aims to extend this research by investigating GLUT expression in diabetic mouse models using both qPCR and immunochemistry. We aim to determine what regulates GLUT expression in sympathetic neurons and how expression may be affected in hyperglycaemic conditions.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): The final grade will be based on an evaluation of laboratory performance (50%) and a final written report (50%) by the supervisor.

Project status: This project is taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects; Handling chemicals.