'Phototoxicity and pulsed laser illumination' - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Claire Brown

Supervisor's Email: claire.brown [at] mcgill.ca

Supervisor's Phone: 541-677-7493

Supervisor's Website: https://mcgill.ca/physiology/directory/core-faculty/claire-brown

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Phototoxicity and pulsed laser illumination

Project description (50-100 words suggested): It has recently been shown that using pulsed laser light reduced photobleaching and phototoxicity in fluorescence microscopy and live cell imaging. This has the benefit of preserving fluorescence during light intensive confocal laser scanning microscope (CLSM) imaging. It also has the benefit of preserving living samples during live cell timelapse imaging. This project will test the effect of different scanning configurations on the CLSM on phototoxicty to living cells. The student will learn how to use the confocal microscope, image living cells, collect timelapse movies and perform image processing and analysis to track cells and to measure photo-toxicity.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% for the laboratory work and 50% for the written report.

Project status: This project is taken.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: None of the above (NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers).