'Assessing intestinal fibrosis in a mouse infection model' - MIMM 397 Undergraduate Research Project Application Form

Supervisor's Name: Samantha Gruenheid

Supervisor's Email: samantha.gruenheid [at] mcgill.ca

Supervisor's Phone: 514-398-2138

Supervisor's Website: www.mcgill.ca/mrcct/members/primary-members/samantha-gruenheid

Supervisor's department: Microbiology and Immunology

Course number: MIMM 397 (Immunology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Assessing intestinal fibrosis in a mouse infection model

Project description (50-100 words suggested): Our lab has identified a specific mouse strain which is highly susceptible to infection with the intestinal pathogen Citrobacter rodentium. This leads to rapid mortality following infection with virulent C. rodentium strains. In further characterizing this model, we have found that when these same highly susceptible mice are infected with an attenuated pathogen (one that is harmless in normal mice), they develop a striking thickening of the cecum and proximal large intestine. We hypothesize that this is due to the development of intestinal fibrosis. The student will test this hypothesis by assaying tissues from infected and control mice for collagen deposition (a hallmark of fibrosis), using the Sircol collagen assay.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).

Other project information: The student will not work directly with live animals, only with tissues.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply / Next steps: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects; Biohazardous substances.