'Novel regulators of HAQ biosynthesis' - MIMM 396 Undergraduate Research Project Application Form

Supervisor's Name: Dao Nguyen

Supervisor's Email: dao.nguyen [at] mcgill.ca

Supervisor's Phone:

Supervisor's Website:

Supervisor's department: Microbiology and Immunology

Course number: MIMM 396 (Microbiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2013

Project end date: Friday, April 11, 2013

Project title: Novel regulators of HAQ biosynthesis

Project description (50-100 words suggested): Several potential novel regulators of a family of molecules called 4-hyrdorxy-alkyl-quinolines were identified in a random transposon mutagenesis screen of Pseudomonas aeruginosa. For this project, the student will confirm the location of the transposon insertions in mutant clones and determine whether the candidate genes identified in the genetic screen are involved in regulation of HAQ biosynthesis. This project will involved PCR and sequencing of mutations, and characterization of gene expression using transcriptional LacZ reporters.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply / Next steps: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances; Handling chemicals.