Genomic imprinting during meiosis - HGEN 396 Undergraduate Research Project Application Form

Supervisor's Name: Anna Naumova

Supervisor's Email: anna.naoumova [at] mcgill.ca

Supervisor's Phone: 514-934-1934 x 35906

Supervisor's Website:

Supervisor's department: Human Genetics

Course number: HGEN 396 (Human Genetics)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Genomic imprinting during meiosis

Project description (50-100 words suggested): Genomic imprinting is an epigenetic mechanism that causes monoallelic parent-of-origin dependent gene expression in mammals.  Genomic imprints are reset during gametogenesis to ensure normal development of the future mammalian embryos.  However, little is known about the exact timing of imprint resetting and transcriptional regulation of imprinted genes during meiosis. Several studies suggest that imprint resetting is complete before the onset of meiosis. Our preliminary data suggest that completion of resetting does not occur until the onset of the first meiotic division. The goal of this project is to refine the timing of imprint establishment during spermatogenesis. Completion of imprint establishment is expected to cause biallelic expression of imprinted genes.  The student will analyze allelic expression of four imprinted genes in mouse testis to determine the stage when their expression becomes biallelic (e.g. genes are expressed from both chromosomes).  This work will require conducting RNA extraction, reverse transcription and PCR, running agarose gels and analyzing the results.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor. Details of the evaluation scheme will be provided by HGEN to supervisors and applicants.

Other project information: The student should have a good knowledge of basic chemistry, genetics, and molecular biology, manual dexterity and enthusiasm for research.  The project will require mouse handling.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply / Next steps: For HGEN: After all of the parts of this application forms are completed and the hard copy is signed by the professor and the student, bring the application form and a copy of your unofficial transcript to the Department of Human Genetics (to the attention of Dr. Patricia N. Tonin, Stewart Biology Building N5.13) during office hours.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects.