Establishing an RNAi-based screen for factors that regulate Polycomb protein binding in mitosis - BIOC 396 Undergraduate Research Project Application Form

Supervisor's Name: Jacques Drouin / Nicole Francis

Supervisor's Email: nicole.francis [at]

Supervisor's Phone: 514-987-5558

Supervisor's Website:

Supervisor's department: Biochemistry

Course number: BIOC 396 (Biochemistry)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Establishing an RNAi-based screen for factors that regulate Polycomb protein binding in mitosis

Project description (50-100 words suggested): Polycomb Group proteins regulate gene expression through epigenetic effects on chromatin, but how their effects are inherited through mitosis is not known. In mitosis, binding of Polycomb proteins to chromatin is dramatically reduced. The mechanisms governing this release must be coupled to the molecular events that drive mitosis. To identify factors that are required for release of Polycomb proteins, RNAi will be carried out in Drosophila S2 cells against a collection of genes that regulate mitosis and binding of Polycomb proteins to mitotic chromosomes assayed. RNAi against some mitotic regulators is expected to interfere with release of Polycomb proteins in mitosis.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% final report, 50% lab performance (including attendance, technical performance, independence, preparedness/understanding of the project, and notebook).

Project status: This project is taken.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.