'Analysis of PIP3 and TBC-2 membrane recruitment' - ANAT 396 Undergraduate Research Project Application Form

Supervisor's Name: Christian Rocheleau

Supervisor's Email: christian.rocheleau [at] mcgill.ca

Supervisor's Phone: 514 398-3979

Supervisor's Website: www.rocheleaulab.com

Supervisor's department: Anatomy and Cell Biology

Course number: ANAT 396 (Anatomy and Cell Biology)

Term: Fall 2013-2014

Project start date: Tuesday September 3, 2013

Project end date: Monday, December 16, 2013
The student must present his/her final report EITHER: by the last day of classes (December 3, 2013); OR, subject to the following conditions, no later than the last day of the final exam period. Conditions to end the project during the exam period: that submissions can be reviewed and grades submitted by the appropriate deadline, and also that the workload required to complete the project not exceed that normally required to prepare for final examinations.

Project title: Analysis of PIP3 and TBC-2 membrane recruitment

Project description (50-100 words suggested): The Rab5 and Rab7 GTPases are important regulators of endosome maturation. TBC-2 is a Rab5 GTPase Activating Protein that is recruited to endosomes to inactivate Rab5. However we do not yet know the mechanisms by which TBC-2 is recruited, however the presence of a PH lipid binding domain suggests that endosomal PIP3 might be important. The student will test the recruits for PI3 kinases and phophatases for roles in recruitment of TBC-2 to endosome membranes using genetic approaches and confocal microscopy.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation by the supervisor of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%).

Project status: This project is taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances.