'Effect of nicotine on the chick embryo brain' - NSCI 396 Undergraduate Research Project Application Form.

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it from www.mcgill.ca/science/research/ours/396 after it has been posted. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature). Ask your supervisor to sign her/his section near the bottom. Take it to the department corresponding to the course number; this may or may not be your own department. Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102. The Office for Undergraduate Research in Science will later post the project online at www.mcgill.ca/science/research/ours/396/listing, indicating whether the project is open for students to apply or taken.

QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964

Supervisor's Name: Maria Pompeiano

Supervisor's Email: maria.pompeiano [at] mcgill.ca

Supervisor's Phone:

Supervisor's Website:

Supervisor's department: Psychology

Course number: NSCI396 (Neuroscience)

Term: Fall 2011-2012

Project start date: Wednesday, September 1, 2011

Project end date: Tuesday, December 6, 2011

Project title:Effect of nicotine on the chick embryo brain

Project description:

Acetylcholine is one of the main neurotransmitters implicated in developmental regulation and later in the regulation of the sleep-waking cycle.

Nicotine mimics the action of ACh on a subset of receptors called nicotinic-cholinergic receptors. The presence of ACh in high levels at nicotinic receptors in the embryo brain suggests that brain cells maybe responsive to nicotine already at early stages of development and could be affected by exposure to nicotine during critical developmental periods. We are going to treat chick embryos at a specific age with nicotine. There is a reason to believe that this could interfere with the normal establishment of synaptic connection and can thus deeply effect the general organisation of the developing and mature brain.

We will study the effect of nicotine on the expression of particular target genes of interest (transcription factors, channels, etc.) by using immunohistochemistry on brain slices of chick embryos.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% report; 50% lab performance

Other project information: The student will learn how to handle chick embryos, to perfuse embryos, to cut embryo brains at the cryostat, as well as  immunohistochemical techniques

Project status - This project is: Taken.

How students can apply: Bring a printed copy of this application form and your advising transcript to me during office hours.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. Which of the following, if any, is involved? Animal subjects; Handling Chemicals.

Student's Name:

Student's McGill ID:

Student's Email (first.last [at] mail.mcgill.ca):

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:

Date: 

Unit chair/director/designate's name: 

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses:

Date: