'The effects of chronic pain on the glial response in the brain' - NSCI 396 Undergraduate Research Project Application Form.

INSTRUCTIONS - PROFESSORS: Please review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it from www.mcgill.ca/science/research/ours/396 after it has been posted. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature). Ask your supervisor to sign her/his section near the bottom. Take it to the department corresponding to the course number; this may or may not be your own department. Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102. The Office for Undergraduate Research in Science will later post the project online at www.mcgill.ca/science/research/ours/396/listing, indicating whether the project is open for students to apply or taken.

QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964

Supervisor's Name: M. Catherine Bushnell, Ph.D.

Supervisor's Email: catherine.bushnell [at] mcgill.ca

Supervisor's Phone: 514-398-3493

Supervisor's Website:

Supervisor's department: Neuroscience

Course number: NSCI396 (Neuroscience)

Term: Fall 2011-2012

Project start date: Wednesday, September 1, 2011

Project end date: Tuesday, December 6, 2011

Project title: The effects of chronic pain on the glial response in the brain

Project description:

This project will be investigating the glial response in the rat brain 5 months after nerve injury. Chronic pain causes long-lasting changes in brain structure and function that is not well characterized at this stage. Theories include that chronic nerve damage causes a neuroinflammatory response in the brain, which may be mediated by glial cells that are proliferating and impacting upon structure and function.

During the course of this project, brain tissue that has been paraffin-embedded and sectioned will be immunohistochemically stained for GFAP (Glial fibrilliary acidic protein), a marker for astrocytes (a type of glia). Fluorescent and DAB (diaminobenzidine) visualization will be performed, and glial staining localized and quantified in regions of interest in the brain, including prefrontal, motor, sensory and limbic cortices. Image processing will be performed using ImagePro software, and cell counts and intensity levels quantified. Statistical analysis will be performed using GraphPad Prism software, and results incorporated into a manuscript for publishing.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 40% Lab component, 60% report.

Other project information:

Project status - This project is: Taken. The professor has no more '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. Which of the following, if any, is involved? Handling chemicals

Student's Name:

Student's McGill ID:

Student's Email (first.last [at] mail.mcgill.ca):

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student signature - I have not applied for another '396' course in this term:

Supervisor's signature:

Date:

Unit chair/director/designate's name:

Unit chair/director/designate's signature:

Date: