'The role of puberty in consolidation of sex-specific expression in heart' Undergraduate Research Project Application Form.

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Lorraine Chalifour
Email: lorraine.chalifour [at] mcgill.ca (lorraine dot chalifour at mcgill dot ca)
Phone: 514-340-8222 ext4295
Supervisor's department: None of the above
Supervisor's department (if none of the above) Medicine, Experimental Medicine
Course number: MIMM396 (Microbiology)
Term: Winter 2010-2011
Project start date: January 4 2011
Project end date: April 8 2011
Project title: The role of puberty in consolidation of sex-specific expression in heart.
Project description: The expression of proteins involved in calcium homeostasis is sex specific in heart. Mature adult females have a significantly greater abundance of NCX1 and calsequestrin than males and a reduced abundance of calreticulin and pS16-PLB. These differences are reversed post gonadectomy indicating that maintenance of sex hormones is necessary for continued sex specific expression. In normal mice, we found sex specific expression of calsequestrin in hearts of day 2 neonates indicating sex specific expression is evident very early. Fetuses are exposed to hormones in utero and we showed that gestational delivery of the feminizing chemical, diethylstilbestrol or masculinizing chemical, testosterone proprionate significantly altered expression in the mature adult especially SERCA2a and calsequestrin. Our working hypothesis in this study is that expression is initially set to be sex specific in utero but that the hormonal changes present in hypertrophy are necessary to consolidate sex specific expression. In current study, we treated fetal mice with either vehicle or DES on gestational days 11-14. At weaning, some of the mice were left intact, some were ovariectomized at weaning (before puberty) and others ovariectomized when mature (8 weeks). The expression of proteins involved in calcium homeostasis ie. NCX1, SERCA2a, Calsequestrin, phospholamban the phosphophospholamban will be measured in heart homogenates by immunoblots. We expect the expression pattern in females gonadectomized when mature to have predicted changes in expression when compared with intact females. We expect females gonadectomized before puberty to have a significantly different pattern and resemble the pattern of gonadectomized males. We expect the DES treated females to show blunted increases in SERCA2a and calsequestrin. The results from these studies will help us to understand the role of pubertal hormones in consolidating the sex specific programming initiated during gestation.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%)."
Other project information: Animal samples have already been prepared so there is no need for animal training and handling experience.
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [ ]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:  
Email (first.last [at] mail.mcgill.ca):  
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):  
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: