'Study of neuronal cell navigation on molecular landscapes at the nanoscale.' Undergraduate Research Project Application Form.

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science.

QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.


Name: David Juncker

Email: david.juncker [at] mcgill.ca (david dot juncker at mcgill dot ca)

Phone: 514-398-7676

Website: https://wikisites.mcgill.ca/djgroup

Supervisor's department: Biomedical Engineering

Course number: ANAT396 (Anatomy and Cell Biology)


Term: Winter 2010-2011

Project start date: January 4, 2011

Project end date: April 8, 2011

Project title: Study of neuronal cell navigation on molecular landscapes at the nanoscale.

Project description: Neuron guidance is a key process in development that leads to the creation of the proper connections within the mature nervous system. This process was determined to be monitored by the expression of various guidance proteins that affect the neuron, as it grows, in a variety of ways. In most cases, the understanding behind the mechanism that results in the neuronal response remains unclear. To better study the effects of these molecules on the neurons, a number of techniques have been developed to create protein patterns in vitro. To investigate the role of these proteins, the Micro-and Nano Bioengineering Lab Is using micro contact printing (µCP), a stamping technique, to print patterns of a variety of proteins onto glass slides. These micropatterns are then used to investigate the affect on neuronal growth over extended periods of time. The student will generate patterns of various proteins and examine the resulting neural growth response using timelapse microscopy.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Other prerequisite, if applicable:

Grading scheme (The final report must be worth at least 50% of final grade): lab notebook 15%, biweekly progress reports, 10%, final report 50%, final presentation 25%

Other project information: Techniques Used:
-Protein Micropatterning using soft lithography
-Cell culture
-Light microscopy, fluorescence microscopy and live imaging
-Image analysis (Image J, Matlab?)

Project status - This project is: Taken. The professor has no more '396' projects this term.

How students can apply: (contact by email for meeting) N/A, this project is taken.

Ethics, safety, and training

Which of the following, if any, is involved? One or more of the following

Animal subjects [ ]

Human subjects [ ]

Biohazardous substances [x]

Radioactive materials [ ]

Handling chemicals [x]

Using lasers [ ]

Supervisors are responsible for the ethics and safety compliance of undergraduate students.


Do not complete this section unless/until the student is identified.


McGill ID:

Email (first.last [at] mail.mcgill.ca):


Program (E.g., B.Sc. Maj. Chem. Min. Biol.):

Level (U0 / U1 / U2 / U3):

Student signature - I have not applied for another '396' course in this term:


Do not complete this section unless/until the student is identified.

Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.

Supervisor's signature:


Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.

Unit chair/director/designate's name:

Unit chair/director/designate's signature: