‘396’ Undergraduate Research Project Proposal Form

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102.

QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.

Name: Edward Ruthazer
Email: edward.ruthazer [at] mcgill.ca
Phone: 514-398-4022
Website: http://ruthazerlab.mcgill.ca
Supervisor's department: Anatomy and Cell Biology
Supervisor's department (if none of the above) Neurology and Neurosurgery
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Winter 2008-2009
Project start date: January 5 2009
Project end date: April 14 2009
Project title: Transneuronal transfer of Rab5 mutants by expression of TAT-fused expression vectors
Project description: 1. test new peptide delivery technique: TAT-fused GFP will be expressed by electroporation in Xenopus optic tectum to
determine if this is an efficient method to deliver proteins to all the cells in a targeted brain structure. RFP will be
coexpressed as a control.
2. Apply new technique to study retrograde transsynaptic effects of synaptic LTD: The effects of overexpression of a
Rab5 dominant negative mutant in tectal neurons, which will prevent internalization of AMPA receptors at synapses,
will be studied by repeated imaging of GFP-expressing axons innervating the affected tectal area. Controls will
include cells expressing inert TAT-peptides and presynaptic expression of the Rab5dn construct without the TAT tag.
3. Some of these constructs will have to be made by subcloning of existing constructs.
All the above will be carried out under the supervision of a PhD student Neil Schwartz and the PI Ed Ruthazer.
Prerequisite: 1 term completed at McGill + CGPA ≥ 3.0; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): The student will be assessed for the effort based on bi-weekly meetings with P1 (50%) and Finla Report (50%)
Other project information:
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [x]
Human subjects [ ]
Biohazardous substances [x]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [x]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:  
Email (first.last [at] mail.mcgill.ca):  
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: