396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Marko Horb
Email: marko.horb [at] mcgill.ca
Phone: 514-987-5629
Website: www.ircm.qc.ca/horb
Supervisor's department: Anatomy and Cell Biology
Supervisor's department (if none of the above)  
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Winter 2008-2009
Project start date: January 12, 2009
Project end date: April 14, 2009
Project title: Pancreas development in Xenopus
Project description: Ptf1a is a basic helix-loop-helix (bHLH) transcription factor complex that is essential for development of both endocrine and exocrine pancreas. In order to identify downstream targets of Ptf1a a microarray analysis was performed between control pancreatic tissue and pancreatic tissue lacking Ptf1a in Xenopus. This analysis revealed that a partial knockdown of Ptf1a function resulted in a complete loss of acinar cells, but had no effect on endocrine cells, while a complete knockdown affected both endocrine and exocrine cells. Another microarray analysis was performed between control pancreatic tissue and pancreatic tissue over-expressing Ptf1a in Xenopus. This analysis revealed that over-expression of the transcription factor resulted in the amplification of acinar and endocrine cells, while over-expression of a VP16 fusion protein resulted in a similar amplification of acinar cells, but endocrine cell expression was lost. The focus of this project will be to identify the roles of various candidate genes identified by the microarrays. Techniques involved include quantitative PCR and in situ hybridization.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): 50% lab research and 50% written report
Other project information:
Project status - This project is: Taken; however students may contact the professor to discuss other possible '396' projects this term.
How students can apply: Other
If other, please specify: email
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [x]
Human subjects [ ]
Biohazardous substances [ ]
Radioactive materials [ ]
Handling chemicals [ ]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:
Email (first.last [at] mail.mcgill.ca):
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: