Undergraduate Research Project Proposal Form

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Prof. John A. DiBattista
Email: john.dibattista [at] mcgill.ca (john.dibattista) -at-mcgill.ca
Phone: 514-934-1934 ext. 34401
Supervisor's department: Anatomy and Cell Biology
Supervisor's department (if none of the above)  
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Winter 2008-2009
Project start date: January 5, 2009
Project end date: April 14, 2009
Project title: Prostaglandin E2-mediated positive feedback regulation of cyclooxygenase-2 gene expression
Project description: The focus of the research is on elucidating the molecular factors and signaling mechanisms implicated in prostaglandin E2 (PGE2)-mediated gene regulation in inflammatory contexts. We have established that PGE2 regulates the expression of many genes, including that of the rate-limiting enzyme implicated in its biosynthesis (i.e. cyclooxygenase-2; COX-2), at the post-transcriptional/translational levels. To date, we have established that overexpression of the RNA binding protein tristetraprolin (TTP) potently reduces COX-2 mRNA stability/translation and that TTP's subcellular localization and activity are modulated by PGE2.
The assigned research project will consist of using basic molecular and cellular biology techniques (i.e. cell culture, gene silencing by shRNA, western blotting) to silence endogenous TTP expression and analyze the effects on exogenous (i.e. transfected) and endogenous COX-2 mRNA stability/translation.
Prerequisite: 1 term completed at McGill + CGPA ≥ 3.0; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): Final Report (50%), Practical Assessment (30%), Oral presentation of project and acquired data (20%).
Other project information:
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [x]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:  
Email (first.last [at] mail.mcgill.ca):  
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):  
Level (U0 / U1 / U2 / U3):
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: