396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Fiona Bedford
Email: fiona.bedford [at] mcgill.ca
Supervisor's department: Anatomy and Cell Biology
Supervisor's department (if none of the above)  
Course number: ANAT396 (Anatomy and Cell Biology)
Term: Winter 2008-2009
Project start date: January 5, 2009
Project end date: April 14, 2009
Project title: The Role of the GDP-GTP Exchange Factor Collybistin in Neurite Outgrowth
Project description: Collybistin is a GDP-GTP Exchange Factor (GEF) for CDC42 that is responsible for the translocation of gephryrin to submembrane microagregates by direct binding of gephryin at collybistin's SH3 domain. Gephyrin in turn is an adaptor protein that binds amongst other things the heterophilic adhesion protein Neuroligin-1. Recently Dr Bedford's group has shown Neuroligin-1 can direct neurite outgrowth in the neuroblastoma cell line N1E-115. This novel function for Neuroligin-1 is likely to play an important role in its ability to control synapse formation. Understanding the molecular determinants of this function will help to confirm this. The role of the small RhoGTPase CDC42 in directing the actin rearrangements required for neurite outgrowth are now well established, therefore finding a CDC42-GEF associated with Neuroligin suggests a potential molecular mechanism by which Neuroligin-1 can control neurite outgrowth. In this project the student will be addressing whether Collybistin through its exchange activity is required for the neurite outgrowth function of Neuroligin-1. Mutant Neuroligin-1 constructs where the collybistin-gephyrin interaction site is mutated as well as several Collybistin GEF-mutants will be cloned and transfected into the N1E-115 cell line. The phenotypes of these mutants will be studied in order to investigate the role of collybistin in neurite outgrowth.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Other prerequisite, if applicable:  
Grading scheme (The final report must be worth at least 50% of final grade): 50% attendance, 50% written report
Other project information:
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [x]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:
Email (first.last [at] mail.mcgill.ca):
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):
Level (U0 / U1 / U2 / U3):
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: