MIMM 397: Identification of a candidate tumor suppressor gene in prostate cancer. (Prof. J. Lapointe)

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
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QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Dr. Jacques Lapointe
Email: jacques.lapointe [at] mcgill.ca
Supervisor's department: None of the above
Supervisor's department (if none of the above) Surgery
Course number: MIMM397 (Immunology)
Term: Fall 2009-2010
Project start date: September 1, 2009
Project end date: December 3, 2009
Project title: Identification of a candidate tumor suppressor gene in prostate cancer
Project description: Genomic deletion of tumor suppressors is a frequent event in cancer progression. In tumors with a single copy deletion of a tumor suppressor, the remaining allele is often mutated or silenced via CpG island hypermethylation within its promoter region. Recently, we have reported a novel genomic deletion in prostate cancer tumors by array-based comparative genomic hybridization (CGH). We have identified a candidate tumor suppressor gene residing in the deleted region whose expression is reduced in heterozygous deletion cases. Mutational analysis of exonic DNA from the remaining allele has shown no mutation. Preliminary data based on bisulfite sequencing, a method to detect promoter hypermethylation, has revealed a primary prostate tumor with a single copy deletion showing hypermethylation. We hypothesize that the remaining allele of the novel candidate tumor suppressor gene is silenced through hypermethylation. We propose to screen more tumors for the heterozygous deletion using fluorescence in situ hybridization (FISH) on formalin fixed paraffin embedded (FFPE) prostate tissue. Genomic DNA from prostate tumors containing the heterozygous deletion will be bisulfite sequenced using a protocol designed for FFPE tissue. Results from these experiments should support the relevance of the candidate tumor suppressor gene in prostate cancer and provide the groundwork for future functional studies.
Prerequisite: Other: please specify below
Other prerequisite, if applicable: Permission of instructor
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).
Other project information:
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
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Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
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Using lasers [ ]
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