MIMM 396 Undergraduate Research Project Proposal Form: Impact of estrogenizing compounds on cardiomyocyte differentiation (Prof. L. Chalifour)

INSTRUCTIONS - PROFESSORS: Fill out Sections A & B then submit this form online. (You will receive an email copy of the form. The Office for Undergraduate Research in Science will also post the project online, indicating whether the project is open for students to apply or taken.) DONE
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted. Either way, print this form. Complete and sign Section C on the hardcopy. Ask your supervisor to sign Section D. Take it to the department corresponding to the course number in Section A (this may or may not be your own department). Do not register for a '396' course on Minerva until you receive departmental permission.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, notify student. If approved, please give student permission to register on Minerva, and fax this form (with signatures) to the Office for Undergraduate Research in Science at 514-398-8102.
QUESTIONS OR FEEDBACK? Contact Victor Chisholm by email, or phone 514-398-5964.
Name: Dr. Lorraine Chalifour
Email: lorraine.chalifour [at] mcgill.ca
Phone: 514-340-8222
Supervisor's department: None of the above
Supervisor's department (if none of the above) Medicine, Experimental Medicine
Course number: MIMM396 (Microbiology)
Term: Fall 2009-2010
Project start date: September 1, 2009
Project end date: December 3, 2009
Project title: Impact of estrogenizing compounds on cardiomyocyte differentiation.
Project description: Cardiomyocytes have estrogen, progesterone and androgen receptors and respond to steroid hormone agonists. We found very early male and female neonates have different expression levels of proteins important for cardiomyocyte contraction. Our experiments to gestational programm mice with estrogenizing and andogenizing chemicals show that feminization and androgenizing alters heart structure and function. We now want to mechanistically measure the impact of estrogenizing chemicals on cardiomyocyte differentiation. Our experiments will use classic estrogen agonists, (1,7 beta estradiol) and a test agonist, bisphenol A. We will measure the expression of cardiomyocyte structural (beta myosin heavy chain) energy (creatine kinase) and calcium homeostasis (calsequestrin) proteins. We will use the H9c2 cell line which can be differentiated to a cardiomyocyte lineage in vitro. Our experiments will test the hypothesis that feminizing hormones and chemicals alter cardiomyocyte differentiation and that this is reflected in altered protein expression.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Other prerequisite, if applicable:
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).
Other project information:
Project status - This project is: Taken. The professor has no more '396' projects this term.
How students can apply: N/A; this project is filled.
If other, please specify:  
Ethics, safety, and training
Which of the following, if any, is involved? One or more of the following
Animal subjects [ ]
Human subjects [ ]
Biohazardous substances [ ]
Radioactive materials [ ]
Handling chemicals [x]
Using lasers [ ]
Supervisors are responsible for the ethics and safety compliance of undergraduate students.
Do not complete this section unless/until the student is identified.
McGill ID:
Email (first.last [at] mail.mcgill.ca):
Program (E.g., B.Sc. Maj. Chem. Min. Biol.):
Level (U0 / U1 / U2 / U3):
Student signature - I have not applied for another '396' course in this term:  
Do not complete this section unless/until the student is identified.
Supervisor: I give my permission for the student identified in section C to register for this project under my supervision.
Supervisor's signature:  
Unit chair/director/designate: I certify that this project conforms to departmental requirements for 396 courses.
Unit chair/director/designate's name:  
Unit chair/director/designate's signature: