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Advanced Level

If the work involves invasive procedures such as injection, blood collection, euthanasia, surgery and/or being in charge of animals, the material in this section must be well understood. If you are the principal investigator of a research or teaching project, you must also do this section.

When doing the test, make sure that the questions for the Advanced Level are answered before sending it back for grading (the test is requested, received and submitted by email).

Content of Advanced Level:


The absence of pain in the presence of stimuli that would normally be painful.


Please note that the following is for rodent surgery. For other species, training from a user with expertise must be obtained prior to undertaking a procedure.


A total loss of sensation in a part of, or in the entire body, as a result of drug induced depression of local / regional (peripheral) or general (central) nervous tissue.