Molecular Biology

Agilent 2100 Bioanalyzer

The Agilent 2100 Bioanalyzer, a microfluidics-based platform, offers solutions for sizing, quantification and quality control of DNA, RNA and proteins with minimal sample consumption.  A selection of on chip gel electrophoresis kits is available.  High quality digital data includes RIN algorithm (RNA Integrity Number).

RNA Analysis:

-  Total RNA, mRNA and small RNA analysis
-  High sensitivity quality control of RNA samples;
   e.g. analysis of RNA derived from laser microdissected
   tissue samples
-  Quality and concentration of cRNA probes

DNA Analysis:

-  High resolution and quantification of DNA: PCR
   fragments, restriction digest, fragmented DNA

Protein Analysis:

-  Highly sensitive protein concentration, size and purity
-  Protein analysis from cell lysates, column fractions, antibodies and purified proteins

PyroMark Q96 MD and PyroMark Q24

PyroMark Q96 MD and PyroMark Q24 instruments for 96-well or 24-well sequencing plates deliver fast and reliable qualitative  sequencing data simultaneously with quantitative allele frequency information. Single-stranded sequencing templates from PCR products are prepared using the PyroMark Vacuum Prep Workstation which process 24 or 96 samples in parallel.

ADS software supports the assay design. CpG and PyroMark MD software enable the sequence and allele frequency analyses.

Applications:

-  Sequence analysis; About 100 bps of sequencing results
-  Genetic variation studies; -insertion -deletions, SNPs,
   short tandem repeats, variable gene copy number
-  Mutation detection; Known or unknown positions within
   a specific spot, point mutations, multiple mutations,
   insertions/deletions
-  Quantification; Degree of DNA /CpG methylation,
   SNP frequency, loss of heterozygosity.

ViiA 7 Real Time PCR system

The ViiA 7 Real Time PCR system with a 96-well fast block provides a precise quantification.  Multiple detection can be carried out in a single reaction.  Its enhanced fluorescence detection allows the greatest number of dye combinations with accurate and sensitive data analysis. 

Primer Express 3.0 Software supports Taqman assays design

Applications:

-  SyBr Green qRT-PCR
-  Pre-validated or custom qPCR Taqman Assays;
   Gene expression, miRNA, protein expression analysis,
   copy number variation, SNP genotyping analysis,
   mutation detection
-  Pre-validated or custom qRT-PCR gene
   expression arrays