C-BIG Repository: Resources for researchers

The C-BIG Repository offers a wide range of fee-for-services access to equipment and services to the research community. Our team will train you on all the equipment available. The C-BIG Repository will also provide the maintenance and the calibration of all the equipment to ensure a high quality and cost-effective core facility that will facilitate and expedite your research.

Industry rate: cbig.mni [at] mcgill.ca (subject: Website%20enquiry%3A%20Pricing) (Email us for pricing )

EQUIPMENT

New sanitary measures are in place. To consult click here​

To reserve your spot click on the calendar:

calendar icon

For access to our equipment or training, cbig.mni [at] mcgill.ca (email us).

Nanodrop 2000 (Thermo Scientific)

Cost: 0.10$/sample

A spectrophotometer (full-spectrum and UV-Vis spectrophotometers) used to quantify and assess purity of DNA, RNA, protein and more!

We offer training, calibration and maintenance of the spectrophotometer.

Guidelines

Before using it, you should have a training (10-15 minutes) with a member of the C- BIG Repository team.

The training will cover:

  • the use of the nanodrop
  • the cleanup before and after use
  • the software

After training, a password will be given to you to access the nanodrop.

Enspire Multimode Plate Reader (Perkin Elmer)

Cost: Free for now

This plate reader is a high performance, compact and configurable instrument designed with filter/quadmonochromator capabilities. It has an intuitive and ‘easy-to-use’ touch screen to reduce assay start-up times and simplify protocols.

For now, the Enspire plate reader is free to use, but in a near future a price will be determined which will allow us to give you more services, i.e. training and maintenance.

Guidelines

For now, please sign the log in sheet available on the reader. Extra sheets are available next to it.

Before using it, you should have a training with a member of the C- BIG Repository team (the complete training will be available soon).

The training will cover:

  • the use of the Enspire
  • the cleanup before and after use
  • the software

Fluorescence Zeiss Microscope​

Details available soon​

Elipsot

Details available soon

MetaExpress

Details available soon

Z Counter

Details available soon

 

SERVICES

Our services will help you to optimize your experiments (reagents, samples, kits and their costs), to stay focused on your goal and to gain access to our SOPs, GLP techniques, expertise and many more.

Here are some fee-for-services that we offer:

  • Access to multiple and various samples
  • Personalized and private sample collection and storage
  • Storage rentals (freezers and liquid nitrogen tanks)
  • LIMS and storage organization training (TissueMetrix)
  • SOP consultations
  • Arrangement with the appropriate department for consenting and recruiting participants for you area of interest
  • And more.

For questions about services and pricing, cbig.mni [at] mcgill.ca (email us).

 

DOCUMENTATION 

You will find here any supplemental information about the C-BIG Repository, i.e. SOPs, publications, posters.

 

SOPs

If any inquiries please contact us directly at cbig.mni [at] mcgill.ca

 

General:​

  • C-BIG-01-001 Administration of Standard Operating Procedures (CBIG-01-001)​

 

Procedural:​

  • CBIG-02-001 PBMC Isolation from Whole Blood (Conventional Method) (CBIG-02-001)​
  • CBIG-02-002 PBMC Isolation from Whole Blood (Leucosep Method) (CBIG-02-002)​
  • CBIG-02-003 Serum Isolation from Whole Blood (CBIG-02-003)​
  • CBIG-02-004 DNA Extraction from Whole Blood (Accelerated Method) (CBIG-02-004)​
  • CBIG-02-005 DNA Extraction from Whole Blood (Conventional Method) (CBIG-02-005)​
  • CBIG-02-006 DNA Extraction from Saliva (CBIG-02-006)​ 
  • CBIG-02-007 DNA Quantification through NanoDrop Spectrophotometry (CBIG-02-007)​
  • CBIG-02-008 CSF Processing (CBIG-02-008)​
  • CBIG-02-009 Preparation and Testing of Human AB Serum for Subsequent Storage and Use (CBIG-02-009)​
  • CBIG-02-010 Thawing of Cryopreserved Cells (CBIG-02-010)​
  • CBIG-02-011 Plasma Isolation from Whole Blood (CBIG-02-11)
  • CBIG-02-012 Skin Biopsy (Sampling Procedure, Transport and Culture) (CBIG-02-012)​
  • CBIG-02-013 Fibroblast Culture (CBIG-02-013)​
  • CBIG-02-014 DNA Extraction from cell pellet (in preparation)​
  • CBIG-02-015 How to Use an Oragene-DNA Test Tube for the Collection of Saliva (CBIG-02-015)​

 

Equipment:​

 

Forms:​

Publications

Articles:​

Natural killer cells regulate Th17 cells after autologous hematopoietic stem cell transplantation for relapsing remitting multiple sclerosis. Darlington et al. Frontiers in Immunology, 2008​

Posters:​

13th Conference on Biobanking, Vancouver 2019 – Open Science Clinical, Biological, Imaging and Genetic Repository – An Integrated Approach to Biobanking​

BioConnect, Montreal 2016 – Open Science Clinical, Biological, Imaging and Genetic Repository – an Integrated Approach to Biobanking​

Oral Presentations:​

American Academy of Neurology (AAN), Toronto 2020 – Clinical, Biological, Imaging and Genetic Repository C-BIGR; An integrated Approach to Biobanking in the Context of Open Science

 

Canadian Tissue Repository Network logo

Office of Biobank Education and Research logo

The Neuro logo McGill logoMcGill University Health Centre logoKillam logo

 

The Neuro is a McGill research and teaching institute; delivering high quality patient care, as part of the Neuroscience Mission of the McGill University Health Centre. We are proud to be a Killam Institution, supported by the Killam Trusts.

 

 

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