May 7-9, 2019
Jeanne Timmins Amphitheatre
The Neuro
3801 University
The Quantitative Super-Resolution Microscopy Course is targeted at researchers who have experience in advanced optical microscopy and would like to push their expertise into the realm of quantitative imaging in super-resolution microscopy. The goal will be to help researchers develop the practical skills and best practices in this field.
The participants will work on the three most common super-resolution microscopy techniques (single molecule localization microscopy, structured illumination microscopy, and stimulated emission-depletion microscopy) as well as enhanced-resolution confocal microscopy techniques. Good practices and quantitative approaches will be emphasized throughout the super-resolution imaging experiment workflow, including experimental design, image acquisition and analysis.
The course will allow researchers to apply these fairly new techniques in the life sciences with competence and confidence.
The three day course begins on May 7, 2019; the course fee which includes training and meals is $1000.
Program
| May 7 | Super-resolution Fundamentals & Sample Preparation Chair: Claire Brown |
|---|---|
| 8-8:30 | Registration |
| 8:30-8:45 | Welcome and Introductory Remarks, Overview of the Course |
| 8:45-9:20 |
Introduction to the World of Super-Resolution Microscopy |
| 9:20-9:55 |
Sample Preparation for SIM and STED |
| 9:55-10:30 |
Sample Preparation for Single Molecule Localization Microscopy (SMLM) |
| 10:30-11 | Refreshment Break |
| 11-13 | Laboratory Activity #1 |
| 13-14:30 | Lunch (TC Foyer) |
| 14:30-16:30 | Laboratory Activity #2 |
| 16:30-17 | Refreshment Break |
| 17-19 | Laboratory Activity #3 |
| May 8 | Acquiring Quantifiable Data Chair: James Jonkman |
|---|---|
| 8-8:30 | Q&A and Laboratory Review |
| 8:30-9 |
Optimization of Enhanced Resolution Confocal Techniques and STED Setup and Potential Pitfalls |
| 9-9:30 |
Optimization of SMLM Setup and Potential Pitfalls
|
| 9:30-10 |
Optimization of SIM Setup and Potential Pitfalls
|
| 10-10:30 |
New SIM Developments
|
| 10:30-11 | Refreshment Break |
| 11-13 |
Laboratory Activity #4
|
| 13-14:30 | Lunch (JTC Foyer) |
| 14:30-16:30 |
Laboratory Activity #5
|
| 16:30-17 | Refreshment Break |
| 17:30-18:30 |
Keynote Lecture: Developments Towards More Quantitative Super Resolution Microscopy |
| 18:30-20 | Wine & Cheese Reception (TC Foyer) |
| May 9 | Quantitative Data Analysis Chair: Thomas Stroh |
|---|---|
|
8-8:30 |
Q&A and Laboratory Review
|
| 8:30-9 |
Optimization of Deconvolution Techniques and Potential Pitfalls
|
| 9-9:30 |
How to Measure Resolution
|
| 9:30-10 |
Quantitative Analysis from Different Types of Super-Resolution Microscopes
|
| 10-10:30 |
Caution with Post Processing - case studies |
| 10:30-11 |
Refreshment Break |
| 11-13 | Laboratory Activity #6 |
| 13-14:30 | Lunch (JTC Foyer) |
| 14:30-16:30 | Laboratory Activity #7 |
| 16:30-17 | Refreshment Break |
| 17-19 | Laboratory Activity #8 |
| 19-20:30 |
Closing Reception |
Keynote Lecture
Dr. Joerg Bewersdorf will deliver the keynote lecture “Developments towards more quantitative super-resolution microscopy” on Wednesday, May 8, 2019 at 5:30 pm in the Jeanne Timmins Amphitheatre. A reception will follow.
Speaker bio
Joerg Bewersdorf is a Professor of Cell Biology and of Biomedical Engineering at Yale University. He received his Master's degree (Dipl. Phys., 1998) and his doctoral degree in physics (Dr. rer. nat., 2002) training with Dr. Stefan W. Hell at the Max Planck Institute for Biophysical Chemistry in Goettingen, Germany. After 4 years at The Jackson Laboratory in Bar Harbor, Maine, he relocated his research group to Yale University in 2009. An optical physicist/biophysicist by training, Dr. Bewersdorf has been a long-time contributor to the field of super-resolution light microscopy development and the application of these techniques to cell biological questions.
Partners
Hotels
Delta Hotel
475 President-Kennedy Avenue, Montreal, QC H3A 1J7
Description: Set in the heart of downtown Montreal, Delta Hotel is conveniently located minutes from Quartier des spectacles, Centre Bell and Place des Arts. Old Montreal, the city's historic district, is less than 1.2 miles away. Additionally, the Delta Montreal is within 5 minutes’ walk of McCord Museum, which features the history of Montreal.
Reservation: To book your stay at Delta Hotels, please call Marriott Reservations at 1 (844) 860-3753. You must identify yourself as part of the Montreal Neurological Institute and Hospital group staying at the Delta Hotels Montreal (475 President-Kennedy Avenue, Montreal H3A 1J7. A credit card will be required to guarantee your reservation. You are reminded to book by March 14, 2019; any requests made after this date are dependent upon the availability of the rooms and rates.
Room information: The nightly rate for a single or double occupancy standard room is $154.00 (taxes not included). The room includes free WIFI, complimentary bottled water, coffee maker and tea service.
Amenities: Complimentary Wi-Fi, ergonomic workstations with connectivity docks, MP3 docking station, a flat-screen HD TV and 24-hour room service. Other amenities in the hotel include a business center and gym with two squash courts and the Bistro-Bar Le Cordial.
Reservation link: Book your group rate for Montreal Neurological Institute
McGill University has agreements for special rates with several other downtown hotels.
Scientific Committee
Claire Brown
James Jonkman
Thomas Stroh
Erika Wee
Directions
Location
The Montreal Neurological Institute and Hospital is at 3801 University Street, north of Pine Avenue West, on the McGill University campus opposite the former Royal Victoria Hospital.
Montreal is served by highway Routes 10, 15, 20 and 40, and by Greyhound Bus, ViaRail and the P-E-Trudeau airport. In the city, bus and metro service is provided by the Société de transport de Montréal (STM).
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Wheelchair access
A wheelchair accessible entrance is on University Street north of the main entrance. Another wheelchair accessible entrance is in the loading area behind the building: to enter the loading area, turn into the driveway south of the main entrance. Please note, there is no parking in the loading area.
Parking
Parking near the MNI is sometimes difficult. There are parking meters on University Street and a parking lot north of the main entrance. To enter the lot, turn right into the driveway toward Molson Stadium.
Information about parking fees
Taxi Stand
There is a taxi stand on University Street across from the main entrance. You may call a cab from the free taxi phone in the main lobby near the Security Desk.
Access by Public Transportation (STM website)
Bus
There are four bus stops within walking distance:
- Bus 144 stops at Pine Avenue and University Street
- Bus 356 stops at Sherbrooke Street and University Street (Nightbus)
- Bus 107 stops at Pine Avenue and Docteur Penfield
- Bus 24 stops at Sherbrooke Street and University Street
Metro
Take the Metro Green Line to the McGill station. Walk north on University Street and cross Pine Avenue. The main entrance is on the right, past the flags.
Contact
Debbie Rashcovsky
Events Officer
Director's Office
Montreal Neurological Institute & Hospital
3801 University Street Suite 684
Montreal, Quebec H3A 2B4
Tel: 514-398-6047
Cell: 514-971-6047
Email:debbie.rashcovsky [at] mcgill.ca
