on Nikon TE 2000-U inverted microscope (Rm 889 Penfield) |
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Light Sources | Objectives | Fluorescence Filter cubes |
Features |
Halogen lamp (Brightfield) |
Plan Fluor 10×/0.30 Ph I DLL |
DAPI | Cell Fully motorized stage (x,y,z) |
Xenon 100 W (Fluorescence) |
Plan Fluor 20×/0.45 DIC L |
FITC/Cy2/GFP | Ratiometric Ca++ imaging (FURA-2) |
S Fluor 40×/1.30 Oil | TRITC/Cy3/RFP | Sutter Lambda 10-2 filter changer and shuttering system |
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Plan Fluor 60×/0.50 – 1.25 Oil Iris DIC H |
Texas Red | Life cell time lapse imaging |
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Plan Fluor 100×/1.30 Oil Ph3 DLL |
Cy5 | Photometrics Coolsnap HQ cooled CCD |
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Operated by Metamorph and Metafluor software | |||
A separate computer running the same software is available for offline data analysis (Rm 880) |
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Reservation: Signup Sheet at Microscope |