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UID:20261031T142243EDT-6488urC6DA@132.216.98.100
DTSTAMP:20261031T182243Z
DESCRIPTION:The MIAM Trainee Committee presents NATALIE WOO (Physiology) as
  she discusses 'The ExSTED Frontier: Biological Imaging Resolutions Below 
 20 Nanometers.'\n\nUNDERSTANDING NANOSCALE PROTEIN distributions and inter
 actions remains a major challenge in biological imaging due to limitations
  imposed by the diffraction limit of light. Expansion microscopy (ExM) and
  stimulated emission depletion (STED) microscopy offer complementary appro
 aches to surpass this barrier—ExM by physically enlarging biological speci
 mens and STED by selective deactivation of fluorophores to generate a sub-
 diffraction excitation volume. Natalie's research aims to advance the deve
 lopment\, validation\, and biological application of these tools individua
 lly and in combination (ExSTED) to achieve resolutions below 20 nanometers
 . High-expansion factor ExM protocols were optimized to improve mechanical
  stability and broaden applicability\, enabling reliable imaging across di
 verse sample types\, including cell cultures\, thick tissues\, and complex
  biological models. Data will be presented demonstrating a robust\, reprod
 ucible imaging protocol encompassing sample preparation\, immunolabeling\,
  handling and acquisition\, with tools and standards that enhance the acce
 ssibility and reliability of nanoscale imaging in biomedical research.\n\n
 NATALIE WOO is a PhD candidate in Physiology at McGill University\, where 
 she develops and applies cutting-edge bioimaging tools to study cell migra
 tion. Natalie is actively involved in international imaging communities\, 
 including as co-chair of BINA’s Early Career Working Group and cohost of t
 he Expansion Microscopy User Group. Through both her research and leadersh
 ip\, she is committed to advancing imaging accessibility and standardizati
 on across disciplines.\n
DTSTART:20261027T153000Z
DTEND:20261027T163000Z
LOCATION:FDA 3 (Engine)\, Adams Building\, CA\, QC\, Montreal\, H3A 0E8\, 3
 450 rue University
SUMMARY:MIAM Trainee Showcase - The ExSTED Frontier: High-Resolution Biolog
 ical Imaging
URL:https://www.mcgill.ca/miam/channels/event/miam-trainee-showcase-woo
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