In our research work we use a variety of molecular, cellular, histological and immunological tools.
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PCR based molecular markers – we use microsatellite markers, single nucleotide polymorphisms (SNP) and restriction fragment lenght polymorphisms (RFLP) for genotyping. |
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Sanger DNA sequencing is done using Applied Biosystem's 3730xl DNA Analyzer. |
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Expression studies and SNP genotyping using qRT-PCR on an ABI 7500 real-time PCR system. |
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Immunohistochemistry, in situ hybridisation and fluorescence imaging. |
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Fluorescence-activated cell sorting (FACS) using a BD Biosciences LSR II four-laser cell sorting system. |
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We use Flexivent for investigation of respirator mechanics. |
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Multiplex immunoassays on a BioRad BioPlex 200 system. |