Multi-Photon

• Zeiss Axioexaminer upright microscope with electrophysiology settings.

Features:

• Electrophysiology.
• Multi-photon excitation.
• Slice culture imaging.
• Thick sample imaging.
• Localized uncaging or activation.

Light Sources:

• X-Cite 120.
• Direct-coupled multiphoton laser with or without dispersion compensation – Chameleon (Coherent)
• Extended by OPO.
• VIS laser: 458/488/514 nm Argon laser, 543 nm, 594 nm, 633 nm.

Objectives:

• 10X N-ACP, NA=0.3, W
• 40X PLAN APOCHROMAT, NA=1.0, W
• 63X PLAN APOCHROMAT, NA=1.0, W

Fluorescence Cubes:

• Zeiss FS 49 (DAPI)
• Zeiss FS 38 (eGFP-Alexa489)
• Zeiss FS 14 (Alexa546)
• DIC Polarizer

Detectors:

• Three built in PMT detectors
• Two external Non-Descanned Detectors (NDD)

Location:

• Located in Bellini Building, Room 145.