ImageXpressMicro from Molecular Devices is a fully-integrated hardware and software system for automated acquisition and analysis of images for high throughput cell-based screening (High-Content Screening, HCS) or high content analysis (HCA) for basic research. For more details please see the datasheet [.pdf] and short description.
Here are some key features of the system:
- Environment control including temperature, CO2 and humidity for live cell imaging.
- Robotic fluidics for kinetic studies.
- Plate loading robot for walk away operation.
Nikon objectives
- 4x S Fluor 0.20 NA
- 10x Plan Fluor 0.30 NA
- 20x Plan Fluor ELWD 0.45 NA
- 40x Plan Fluor ELWD 0.60 NA
- 60X Plan Fluor 0.85 NA
- 20x S Fluor 0.75 NA
- 40x S Fluor 0.90 NA
| Chroma filter set | Catalog number | Excitation filter | Emission filter | Dichroics |
| DAPI | 49000 | d350/50x | et460/50m | t400lp |
| eGFP/FITC/AF488/etc(cy2) | 49002 | et470/40x | et525/50m | t495lp |
| TRITC/Cy3/etc | 49005 | et545/30x | et620/60m | t570lp |
| Texas Red/AF594 | 49008 | et560/40x | et630/75m | t585lp |
| Cy5/AF 647 | 49006 | et620/60x | et700/75m | t660lpxr |
| CFP/Cyan | 49001 | et436/20x | et480/40m | t455lp |
| YFP/Yellow | 49003 | et500/20x | et535/30m | t515lp |
| Fura2 | 79000 | d340x,v2 | et510/80m | t400lp |
| d380x,v2 | et510/80m | t400lp | ||
| BCECF | 79001 | et440/20x | et535/25m | t518lpxr |
| et495/10x | et535/25m | t518lpxr | ||
| CFP/YFP FRET | 49001/49003 | et436/20x | et535/30m | t455lp |
| 32108b | 32108b | et436/20x | et535/30m | t455lp |
| ND 0.3 |
Location:
- Located in Bellini Building, Room 151.