Core Equipment and Fees

Core Equipment and Fees

*Industry rates - Please call or e-mail for pricing

Equipment

Function

Academic Rates

BD FACSAria™ Fusion 3-Laser 11-Colour, with ACDU (5B/3R/3V) and Hood

50 mW 488 nm Solid State blue laser 100 mW 640 nm Solid State red laser 85 mW 405 nm Solid State violet laser - Class II Type A2 Biosafety Cabinet - Verified to meet NSF-49 standards - Optical filters to detect the following fluorophores: PE-Cy7, PerCP-Cy5.5/PI, PE-TxRed, PE, FITC, APC-Cy7, AF700, APC, BV421/V450, BV510/V500, BV605

Training

Full service

User Fee

$85/hr

Analysis Assistance

$40/hr

Assay Development

$40/hr

Cyan ADP Analyzer

(9 color flow cytometer)

Training

$100/hr (Min. 3hrs)

User Fee

$40/hr

Analysis Assistance

$40/hr

Assay Development

$40/hr

Luminex 100 Multiplex Plate Reader

User Fee

$40/hr

ImageXpress - MetaXpress Micro XL High-Content Imaging System

User Fee

$40/hr

Victor 3 Plate Reader

User Fee

$20/hr

EnSpire Multimode Plate Reader


Configurable Multimode Plate Reader, including
Included standard options:
- Detection technology: absorbance
- Xenon flash lamp
- Shaker with three modes: linear, orbital and double orbital
- Plate height sensor
- Positive identification for filters and mirrors
- Plate ID Barcode readers: all four sides of the plate
- EnSpire software including data analysis package, integrated computer

User Fee $20/hr

ImmunoSpot® S6 ANALYZERS

CTL’s classic ELISPOT analyzers use visible light for imaging single- and dual-color enzymatic ELISPOT counting and analysis; FluoroSpot analyzers facilitate multi-color fluorescent analysis, in addition to Live-Dead-Apoptotic (LDA) cell counting, among many other applications.

User fee 20/hr

NanoDrop™ 2000/2000c Spectrophotometers. Full-spectrum, UV-Vis spectrophotometers to quantify and assess purity of DNA, RNA, Protein and more.

User fee 10c/well

 

To fulfill its main mission as a translational program at the MNI, the ETP has been establishing several platforms that will also serve as core facilities to the MNI and external communities.

 

Core Facility Equipment

Several core instruments are available through the ETP, including:

The BD FACSAria™ Fusion flow cytometer delivers exceptional multicolor performance and ease-of-use. Best-in-class biosafety expertise for a comprehensive advanced cell sorter and biosafety solution. The fully integrated biosafety cabinet meets emerging operator and sample protection requirements as well as global standards for bioprotection.

Click here to reserve time on the FACSAria.

Beckman Coulter Cyan Flow Cytometer (please contact the ETP for special training):

  • 11 standard parameters and 9 colors
  • multiple laser excitation sources to analyze biological cells, beads or microscopic particles
  • pulse processing speed up to 70,000 events/s
  • high acquisition and analysis capability of up to 100,000,000 event data files.

Click here to reserve time on the Beckman Coulter Cyan Flow Cytometer.

Luminex: A microsphere based multiplexing system that allows for intensive protein and gene expression profiling at a fraction of the labor and materials cost. This system has already been used to develop a profile of potential biomarkers in PD-ADS and other patient cohorts studies by multiple MNI and external investigators.
Click here to reserve time on the Luminex.

 

Victor 3: The VICTOR™ 3 is an instrument designed for running cost-effective cellular assays and screening technologies. The multilabel, multi-task plate readers can run multiple assay technologies such as time-resolved fluorescence assays on a single platform. The ETP Victor 3 has an added injector capability of rapid delivery of compounds to particular wells, and also has a heated stage to maintain plates at specific temperatures.
Click here to reserve time on the Victor3.

 

ImageXpress: A rapid, automated cellular image acquisition system. The ImagXpress allows for morphological analysis of the complex cellular structure of neurons and oligodendrocytes. The high throughput capabilities of this integrated hardware and software technology has had an impact on both industry-related and MNI investigator-initiated studies.
Click here to reserve time on the ImageXpress.

 

EnSpire Multimode Plate Reader

EnSpire® Multimode Plate Reader is designed to meet the demands of multi-user research environments, providing high performance technology and flexibility for a range of applications without compromising sensitivity

Detection Method Absorbance, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence, Label-free

Click here to reserve time on Enspire plate reader

ImmunoSpot® S6 UNIVERSAL

CTL Analyzers are specifically designed for use in applications that benefit from morphometric analysis—applications such as ELISPOT assays; bacterial, yeast, stem cell, tumor colony counting, and various plaque and clonogenic assays. CTL Analyzers are manufactured under ISO 9001:2008 standards for the design, assembly, and service of image acquisition units for use in the pharmaceutical and biotechnical industries.

Click here to reserve time on CTL analyzer

NanoDrop 2000

Micro-volume sample size (0.5-2.0μL)
Easy to use—pipet sample directly onto the pedestal
No dilutions required, even for highly concentrated samples
Fast measurement time of less than 5 seconds
Full spectral output (190 - 840 nm)
Preconfigured methods for A260, A280, labeled DNA or Proteins, and much more
User-friendly software that includes custom methods and data export capabilities

Click here to reserve time on Nanodrop