svetlana.komarova [at] mcgill.ca (Email)
Dr. Komarova’s research program is aimed at understanding the regulation of osteoclasts, the cells responsible for bone destruction in diseases such as osteoporosis, rheumatoid arthritis, periodontitis and bone metastases. Osteoclasts are highly active cells, present in bone only in small numbers. Moreover, osteoclasts are terminally differentiated, and therefore do not proliferate in culture. To understand the mechanisms that determine stimulus-specific gene expression in osteoclasts, Dr. Komarova’s laboratory employs high-speed imaging of live cells, quantitative gene expression analysis, as well as mathematical modeling. These studies will provide new insights into the control of bone resorption in health and disease, which may lead to the development of new therapies for treatment of inflammatory, metabolic and metastatic bone disorders. Dr. Komarova completed a M.Sc. degree in physics at the Moscow State University, Russia, and obtained a Ph.D. degree in biophysics in 1996 from the Institute of Theoretical and Experimental Biophysics, Pushchino, Russia. Before joining the Faculty of Dentistry, Dr. Komarova completed postdoctoral training at NASA Ames Research Center, and in the CIHR Group in Skeletal Development and Remodeling, The University of Western Ontario.
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Selected publications over the last 5 years
1. Boraschi-Diaz, JT Tauer, O El-Rifai, D Guillemette, G Lefebvre, F Rauch, M Ferron, SV Komarova (2017) Metabolic phenotype in the mouse model of osteogenesis imperfecta. J Endocrinol Sep;234(3):279-289.
2. K Tiedemann, D Le Nihouannen, J Fong, O Hussein, JE Barralet, SV Komarova (2017) Regulation of osteoclast growth and fusion by mTOR/raptor and mTOR/rictor/Akt. Front Cell Dev Biol May 18;5:54.
3. McKee TJ, Komarova SV (2017) Is it time to reinvent basic cell culture medium? Am J Physiol Cell Physiol. 312(5):C624-C626
4. Steer K, M Stavnichuk, M Morris, SV Komarova (2017) Bone health in patients with hematopoietic disorders of bone marrow origin: systematic review and meta-analysis. J Bone Miner Res 32(4):731-742.
5. Mackay L*, Mikolajewicz N*, Komarova SV, Khadra A (2016) Systematic characterization of dynamic parameters of intracellular calcium signals. Front Physiol 7:525.
6. Xing S, M Grol, P Grutter, SJ Dixon, SV Komarova (2016) Modeling interactions among individual P2 receptors to explain complex response patterns over a wide range of ATP concentrations. Front Physiol 7:294.
7. Boraschi-Diaz I, Komarova SV (2016) The protocol for isolation and cryopreservation of osteoclast precursors from mouse bone marrow and spleen. Cytotechnology. 68(1):105-14.
8. Komarova SV, Safranek L, Gopalakrishnan J, Ou M-J Y, Mckee MD, Murshed M, Rauch F, Zuhr E (2015) Mathematical model for bone mineralization, Front Cell Dev Biol. 3:51.
9. Abdelaziz DM, Abdullah S, Magnussen C, Ribeiro-da-Silva A, Komarova SV, Rauch F, Stone LS (2015) Behavioral signs of pain and functional impairment in a mouse model of osteogenesis imperfecta. Bone 81:400-406
10. Abdelaziz DM, Stone LS, Komarova SV. (2015) Localized experimental bone metastasis drives osteolysis and sensory hypersensitivity at distant non-tumor-bearing sites. Breast Cancer Res Treat. 153(1):9-20.
11. Rafiei S, Tiedemann K, Tabariès S, Siegel PM, Komarova SV. (2015) Peroxiredoxin 4: A novel secreted mediator of cancer induced osteoclastogenesis. Cancer Lett. 1;361(2):262-70. (CIHR)
12. Maria SM, Prukner C, Sheikh Z, Mueller F, Komarova SV. Barralet JE, (2015) Characterization of biomimetic calcium phosphate labeled with fluorescent dextran for quantification of osteoclastic activity. Acta biomaterialia 20; 140–146
13. Kopesky, K Tiedemann, D Alkekhia, C Zechner, B Millard, B Schoeberl, SV. Komarova (2014) Autocrine signaling is a key regulatory element during osteoclastogenesis. Biol Open 3(8): 767-76.
14. GM. Lopez A, S Xing, OM. Maria, G Sadvakassova, JM. LeDue, H Bourque, P Grutter, SV. Komarova (2014) Micro-injury results in threshold properties in response of osteoblast to local membrane indentation. F1000Research 11; 3:162.
15. Abdelaziz DM, Stone LS, Komarova SV. (2014) Osteolysis and pain due to experimental bone metastases are improved by treatment with rapamycin. Breast Cancer Res Treat 143(2):227-37. (CRS)
16. Maria SM, Prukner C, Sheikh Z, Mueller F, Barralet JE, Komarova SV. (2014) Reproducible quantification of osteoclastic activity: Characterization of a biomimetic calcium phosphate assay. J Biomed Mater Res Part B E102(5):903-12
17. Rafiei S, Komarova SV. (2013) Molecular signaling pathways mediating osteoclastogenesis induced by prostate cancer cells. BMC Cancer. 26;13:605.
18. K Tiedemann, I Boraschi-Diaz, I Rajakumar, J Kaur, P Roughley, DP Reinhardt, SV Komarova (2013) Fibrillin-1 directly regulates osteoclast formation and function by a dual mechanism. J. Cell Sci 126(Pt 18):4187-94.
19. JE. Fong, D Le Nihouannen, K Tiedemann, G Sadvakassova, JE. Barralet, SV Komarova (2013) Moderate excess of pyruvate augments osteoclastogenesis. Biology Open 22;2(4):387-95.
20. M Durand, SV. Komarova, A Bhargava, K Li, C Fiorino, N Nabavi, Morris F. Manolson, RE. Harrison, S. J Dixon, SM. Sims, M J. Mizianty, O. Maria, L Kurgan, G Boire, M de Fatima Lucena-Fernandes, AJ. de Brum-Fernandes, (2013) Monocytes from patients with osteoarthritis display increased osteoclastogenesis and bone resorption: the In Vitro Osteoclast Differentiation in Arthritis (IODA) study. Arthritis and Rheumatism;65(1):148-58.
21. Mourskaia AA, Amir E, Dong Z, Tiedemann K, Cory S, Omeroglu A, Bertos N, Ouellet V, Clemons M, Scheffer GL, Park M, Hallett M, Komarova SV, Siegel PM. (2012) ABCC5 supports osteoclast formation and promotes breast cancer metastasis to bone. Breast Cancer Res. 22; 14(6): R149.
22. Ryser M D., Qu Y, Komarova SV. (2012) OPG in bone metastases: mathematical solution to the puzzle. PLoS Computational Biology 8(10): e1002703
23. GM Lopez A, DJ Oliver, PH Grutter, SV Komarova (2012) Deconvolution of calcium fluorescent indicator signal from AFM cantilever reflection. Microscopy and Microanalysis 18(4):808-15.
24. Kaiser J, Lemaire T, Naïli S, Sansalone V, Komarova SV (2012) Calcium fluxes within cortical bone fluid may affect osteocytes mechanosensitivity. Journal of Theoretical Biology 303, 75–86
25. Hussein O, Tiedemann K, Murshed M, Komarova SV (2012) Rapamycin inhibits osteolysis and improves survival in a model of experimental bone metastases. Cancer Letters. 314(2):176-84