In-person class cancellation and work-from-home / Annulation des cours en présentiel et télétravail

Updated: Tue, 03/10/2026 - 17:14
In-person class cancellation and work-from-home / Annulation des cours en présentiel et télétravail. McGILL ALERT! Due to freezing rain all in-person classes and activities on Wednesday, March 11, will be cancelled. Staff are asked not to come to campus tomorrow unless they are required on site by their supervisor to perform necessary functions and activities. See your McGill email for more information.
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ALERTE McGILL! En raison de la pluie verglaçante, tous les cours et activités en présentiel prévus pour le mercredi 11 mars sont annulés. Nous demandons au personnel de ne pas se présenter sur le campus demain, à moins que leur superviseur ne leur demande d’être sur place pour accomplir des fonctions ou activités nécessaires au fonctionnement du campus. Pour plus d’informations, veuillez consulter vos courriels de McGill.
McGill.CA / Cell Imaging and Analysis Network / Microscopy / Light Microscopy

Technical data for SP8

SP8
SP8 - Leica SP8 point-scanning confocal system, on Leica DMI6000B inverted microscope

Microscope: Leica SP8 point-scanning confocal system, on a Leica DMI6000B inverted microscope, fully motorized, with Leica “SuperZ” Galvo stage, four spectral fluorescent light detectors (three PMT, one HyD high sensitivity detector), one transmitted light detector (PMT)

 

Objectives:

Position

Objective

DIC

1

10x/0.4 dry

HC PL APO 1.x/0.40 CS

(prism not installed)

2

20x/0.7 dry

HC PL APO 20x/0.70 CS

DIC

3

40x/0.85 dry

HCX PL APO 40X/0.85 CORR CS,0.11-

DIC

4

40x/1.1 water

HC PL APO 40x/1.10 W CORR CS2

DIC

5

40x/1.3 oil

HC PL APO 40x/1.30 Oil CS2

DIC

6

63x/1.4 oil

HC PL APO 63x/1.40 OIL CS2

DIC

 

Confocal mode: Lasers, no fluorescence emission filters (all spectral detectors):

Lasers (all diode)

Typical fluorophore

405nm, 50mW, DMOD

DAPI

448nm, 40mW, AOTF

CFP

488nm, 20mW, AOTF

AF488, GFP, YFP

552nm, 20mW, AOTF

TRITC, Cy3 Texas Red, RFP

638nm, 30mW, AOTF

AF633, Cy5, FarRedFP

Laser modulation by AOTF (acusto-optic tunable filters), or DMDO (direct modulation of laser intensity)

 

Wide-field fluorescence:

Light source: Leica EL6000 metal-halide lamp

Fluorescence filter cubes, Leica:

Filter set

Excitation filter

Dichroic

Emission filter

Typical fluorophore

I3

BP450-490

510

LP515

AF488, GFP

N2.1

BP515-560

580

LP590

TRITC, RFP

A

BP340-380

400

LP425

DAPI

 

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