Microscope: Applied Precision V4 Blaze OMX system with TIRF option, two Evolve EM-CCD cameras, live cell chamber, DIC optics, in a light-proof enclosure with HEPA-filter, acquisition and analysis workstations
Objectives (only one can be installed at a time):
| Objective |
| 100x/1.4 oil Olympus, API Certified U-PLANAPO |
| 100x/1.49 TIRF oil Olympus, API Certified U-PLANAPO |
Structured Illumination (SIM) and localization microscopy (DLM) modes:
| Lasers (all diode) | Emission filters | Typical fluorophore |
| 405nm, 100mW | BP 436/31 (420-451nm) | DAPI |
| 488nm, 200mW | BP 528/48 (504-552nm) | GFP, AF488 |
| 568nm, 100mW | BP 609/37 (591-619nm) | mCherry, AF568 |
| 642nm, 300mW | BP 683/40 (663-703nm) | AF647 |
Conventional wide-field fluorescence:
Light source: 6-color InsightSSI (solid state illumination) module
Fluorescence filters:
| Excitation Wavelengths | Emission filters | Typical fluorophore |
| 381-399nm | BP 436/31 (420-451nm) | BFP, DAPI |
| 426-450nm | BP 478/35 (460-495nm) | CFP |
| 461-489nm | BP 528/48 (504-552nm) | GFP, FITC, Alexa 488 |
| 505-515nm | BP 541/22 (530-552nm) | YFP, Alexa 532 |
| 563-588nm | BP 609/37 (590-618nm) | mCherry, Alexa 568 |
| 621-643nm | BP 683/40 (663-703nm) | Cy5, Alexa 647, 635, 660 |