| Multiplexing | The labeled samples are mixed and then separated on the same 2-D PAGE gel. Thus, for samples on the same DiGE gel, gel-to-gel variation is completely eliminated, and the number of gels needed for one experiment can be cut two- to three-fold. |
| Gel-to-gel comparison | One of the three samples on a DiGE gel can be a mixture of equal amounts of all experimental samples, a “pooled internal standard”. This creates a standard for each protein in the analysis. Therefore, comparisons across different gels can be made with a high degree of confidence |
| User-friendly manipulation | Large format gels are cumbersome to handle. Since in DiGE the proteins are pre-labeled, DiGE gels do not have to be manipulated after electrophoresis. Additionally, the scanner that is used for imaging accepts gel sandwiches including the glass plates. This further reduces the variation between gels, and the risk of damaging or destroying gels |