The main interest of the laboratory lies in the immunology of Leishmania infections, focusing on CD8 T-cell biology. Two aspects of CD8 T-cell biology are currently investigated:
- The development of effector and memory CD8 T-cell responses in the experimental model of Visceral Leishmaniasis (VL).
- Priming of CD8 T-cell responses during acute VL.
The laboratory investigates antigen-specific CD8 T- cell responses and host/pathogen interactions using transgenic Leishmania donovani parasites expressing Ovalbumin as a model antigen. We are particularly interested in the early priming events, the requirements for induction and maintenance of effector CD8 T-cell responses, and the generation of memory cells. The experimental model for VL is a great model to investigate the dynamic of in vivo generated CD8 T- cell responses in a setting of chronic infection.
Bankoti, R., Gupta, K, Levchenko, A., and Stäger,S. (2012).
Marginal zone B-cells regulate antigen-specific T-cell responses during infection.Journal of Immunology, in press.
Paun, A., Joshi, T., Pitha, P.M., and Stäger,S. (2011).
IRF5 deficiency severely impairs the development of T helper 1 responses following Leishmania donovani infection.PLoS Pathogens, 7 (1): 1001246.
Ranatunga, D., Hedrich, C.M., Wang, F., McVicar, D.W., Nowak, N., Joshi, T., Fiegenbaum, L., Grant, L.R., Stäger,S., and Bream, J.H. (2009).
A human IL10 BAC transgene recapitulates myeloid- but not T cell-specific murine IL-10 expression resulting in differential disease outcomes.,/a>PNAS,106(40): 17123-28.B7-H1 blockade increases survival of dysfunctional CD8+ T-cells and confers protection against Leishmania donovani infections.PLoS Pathogens,5(5):e1000431.
Joshi T, Rodriguez S, Perovic V, Cockburn IA, Stäger S. (2009).
B7-H1 blockade increases survival of dysfunctional CD8(+) T cells and confers protection against Leishmania donovani infections.PLoS Pathog. 2009 May;5(5):e1000431. Epub 2009 May 15.
Maroof, A., Beattie, L., Zubairi, S., Svensson, M., Stäger, S., and Kaye, P.M. (2008).
Posttranscriptional regulation of Il10 gene expression allows natural killer cells to express immunoregulatory function.Immunity, 29(2):295-305.
Stäger, S., Maroof, A., Zubairi, S., Sanos, S.L., Kopf, M., and Kaye, P.M. (2006)
Distinct roles for IL-6 and IL-12p40 in mediating protection against Leishmania donovani and the expansion of IL-10+CD4+ T-cells.European Journal of Immunology.
Polley, R.*, Stäger, S.*, Prickett, S., Maroof, A., Zubairi, S., Smith, D.F., and Kaye, P.M. (2006)
Adoptive immunotherapy against experimental visceral leishmaniasis with CD8+ T cells requires the presence of cognate antigen.Infection and Immunity, 74(1): 773-776.
* both authors contributed equally
Engwerda, C.R., Ato, M., Stäger,S., Alexander C., and Kaye, P.M. (2004)
Distinct roles for lymphotoxin-β and TNFα in the control of Leishmania donovani infection.American Journal of Pathology, 165(6):2123-2133.