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UID:20260522T054723EDT-5593U6g7D7@132.216.98.100
DTSTAMP:20260522T094723Z
DESCRIPTION:Dr. Martin R. Larsen\nUniverstiy of Southern Denmark\nMcIntyre 
 Medical Building\, Room 521\nPhosphoproteomics\nWe have developed robust s
 trategies for the specific enrichment\nof phosphorylated peptides from com
 plex mixtures\, including\ntitanium dioxide procedures (1) and procedures 
 for the separation\nof multiphosphorylated peptides from monophosphorylate
 d peptides\n(SIMAC)(2). These strategies we have applied to a variety of\n
 biological systems including depolarization signaling in nerve\nendings.\n
 During the last few years we have performed comprehensive\nmapping of phos
 phorylation sites in proteins involved in synaptic\nvesicle endocytosis (S
 VE) which is the mechanism with which\nsynaptic vesicles are recruited bac
 k into the nerve end after\nrelease of neurotransmitters following depolar
 ization of nerve\nendings. The biological events exocytosis and endocytosi
 s are\nextremely important and crucial for maintenance of synaptic\ntransm
 ission between nerve cells.\nNew  strategies has recently been developed f
 or large scale\nphosphoproteomics aiming at downscaling and optimizing the
 \nprocedures for very low amount of materials obtainable from primary\ncel
 ls (µg level).  Preliminary data obtained using one of the\nnew strategies
  on isolated synaptosomes has revealed a large number\nof peptides carryin
 g multiply phosphorylation sites as well as\nsingly phosphorylation sites\
 , which are only detected with this\nnovel technique. We have presently pu
 rified synaptosomes from rat\nbrains and validated the function and qualit
 y of these with respect\nto responses to 10 sec depolarization using 32P-A
 TP incorporation.\nWe have presently identified >2000 unique phosphorylati
 on sites\nfrom very small amount of proteins from synaptosomes using only 
 the\nnew strategy.\n
DTSTART:20090930T180000Z
DTEND:20090930T180000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Phosphoproteomics
URL:https://www.mcgill.ca/channels/event/phosphoproteomics-109646
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