Multi-Photon

  • Zeiss Axioexaminer upright microscope with electrophysiology settings.


Features:

  • Electrophysiology.
  • Multi-photon excitation.
  • Slice culture imaging.
  • Thick sample imaging.
  • Localized uncaging or activation.


Light Sources:

  • X-Cite 120 LED
  • Direct-coupled multiphoton laser with or without dispersion compensation – Chameleon (Coherent)
  • VIS laser: 458/488/514 nm Argon laser, 543 nm, 594 nm, 633 nm.


Objectives:

  • 10X N-ACHROPLAN, NA=0.3, W
  • 20X PLAN APOCHROMAT. NA=0.8
  • 40X PLAN APOCHROMAT, NA=1.0, W, DIC, VIS-IR
  • 63X PLAN APOCHROMAT, NA=1.0, W, VIS-IR


Fluorescence Cubes:

  • Zeiss FS 49 (DAPI)
  • Zeiss FS 38 (eGFP-Alexa489)
  • Zeiss FS 14 (Alexa546)
  • DIC Polarizer


Detectors:

  • Three built in PMT detectors
  • Two external Non-Descanned Detectors (NDD)


Location:

  • Located in Bellini Building, Room 143.