High Content Screening

ImageXpressMicro from Molecular Devices is a fully-integrated hardware and software system for automated acquisition and analysis of images for high throughput cell-based screening (High-Content Screening, HCS) or high content analysis (HCA) for basic research. For more details please see the datasheet and short description.


Here are some key features of the system:

  • Environment control including temperature, CO2 and humidity for live cell imaging.
  • Robotic fluidics for kinetic studies.
  • Plate loading robot for walk away operation.


Nikon objectives

  • 4x S Fluor 0.20 NA
  • 10x Plan Fluor 0.30 NA
  • 20x Plan Fluor ELWD 0.45 NA
  • 40x Plan Fluor ELWD 0.60 NA
  • 60X Plan Fluor 0.85 NA
  • 20x S Fluor 0.75 NA
  • 40x S Fluor 0.90 NA

Chroma filter set

Catalog number

Excitation filter

Emission filter

Dichroics

DAPI

49000

d350/50x

et460/50m

t400lp

eGFP/FITC/AF488/etc(cy2)

49002

et470/40x

et525/50m

t495lp

TRITC/Cy3/etc

49005

et545/30x

et620/60m

t570lp

Texas Red/AF594

49008

et560/40x

et630/75m

t585lp

Cy5/AF 647

49006

et620/60x

et700/75m

t660lpxr

CFP/Cyan

49001

et436/20x

et480/40m

t455lp

YFP/Yellow

49003

et500/20x

et535/30m

t515lp

Fura2

79000

d340x,v2

et510/80m

t400lp

   

d380x,v2

et510/80m

t400lp

BCECF

79001

et440/20x

et535/25m

t518lpxr

   

et495/10x

et535/25m

t518lpxr

CFP/YFP FRET

49001/49003

et436/20x

et535/30m

t455lp

32108b

32108b

et436/20x

et535/30m

t455lp

ND 0.3

       

 

Location:

  • Located in Bellini Building, Room 151.