FLIM

  • LSM 710 Confocal Microscope
  • Zeiss AxioObserver fully motorized inverted microscope


Features:

  • 4D Imaging, multi-color, z-stack timelapse.
  • DIC.
  • Fluorescence-lifetime imaging (FLIM).
  • FRET.
  • FRAP.
  • Live cell imaging.
  • Tiling.
  • Multipoint for timelapse of multiwall plates.


Light Sources:

  • X-Cite 120 LED
  • Ar Ion laser 458/488/514 nm 25 mW
  • HeNe Green 543nm 1mW
  • HeNe 594 nm 2 mW
  • HeNe Red 633 nm 5 mW
  • 440 nm pulsed laser - 20 Hz, 50 Hz, 80 Hz pulse rates, <3 nm pulse width
  • 473 nm pulsed laser - 20 Hz, 50 Hz, 80 Hz pulse rates, <3 nm pulse width


Objectives:

  • 10X EC PLAN NEOFLUAR, NA=0.30
  • 20X PLAN APOCHROMAT, NA=0.80
  • 40X C-APOCHROMAT, NA=1.2, W, Korr, UV-VIS-IR
  • 63X  PLAN APOCHROMAT, NA=1.4, OIL, DIC


Fluorescence Cubes:

  • Zeiss FS 49 (DAPI)
  • Zeiss FS 10 (eGFP-Alexa489)
  • Zeiss FS 14 (Alexa546)
  • DIC Polarizer

Optional

  • Texas Red
  • YFP
  • CFP


Location:

  • Located in Bellini Building, Room 151.