Why image in 3D? - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Claire Brown

Supervisor's Email: claire.brown [at] mcgill.ca

Supervisor's Phone: 00795

Supervisor's Website: www.mcgill.ca/physiology/directory/core-faculty/claire-brown

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Why image in 3D?

Project description (50-100 words suggested): At the Advanced BioImaging Facility (ABIF) we often have researchers ask us if they should do they image collection and analysis in 3D. However, we have little data to support the idea that there is a need for 3D data analysis. This project will involved collecting image data sets of endosomal vesicles and filaments for multiple cells in 2D and 3D using laser scanning confocal microscopy. The images will then be analyzed with MetaMorph software in 2D and with Imaris software in 3D to determine if results are similar or not. Control versus treated cells will be used to determine if scientific conclusions will be affected by 2D versus 3D analysis.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% for laboratory activity and weekly reports/meetings and 50% for the written report. The report will follow a journal article format (Summary, Introduction, Materials and Methods, Results, Discussion/Conclusions and References) and will be approximately 10 pages long including figures.

Project status: This project is open to applicant.

How students can apply / Next steps: Email me your unoffical transcripts and CV and a brief statement as to why you are interested in this project.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.