Studies on HIV Integrase Drug Resistance - MIMM 396 Undergraduate Research Project Application Form

Supervisor's Name: Mark Wainberg

Supervisor's Email: mark.wainberg [at] mcgill.ca

Supervisor's Phone: (514) 340-8307

Supervisor's Website: http://www.ladydavis.ca/en/markwainberg

Supervisor's department: Microbiology and Immunology

Course number: MIMM 396 (Microbiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Studies on HIV Integrase Drug Resistance

Project description (50-100 words suggested): The candidate will purify and characterize the enzymatic properties of Integrase enzymes that contain defined drug resistance substitutions and will compare these activities to that of wild-type enzyme. Two different methods will be used. One is a 3' synthetase assay and the other is an integrase strand transfer assay. Efforts will also be made to understand the results in the context of a 3-dimensional structure of the Integrase protein.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final report: 70%; Lab participation: 30%

Project status: This project is taken.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances; Handling chemicals.