'Identification of Staphylococcal Immune Modulatory Ligands Using Transposonomics' - MIMM 397 Undergraduate Research Project Application Form

Supervisor's Name: Joaquin Madrenas

Supervisor's Email: joaquin.madrenas [at] mcgill.ca

Supervisor's Phone: 514-398-3914

Supervisor's Website:

Supervisor's department: Microbiology and Immunology

Course number: MIMM 397 (Immunology

Term: Summer 2013

Project start date: Monday, May 1, 2013

Project end date: Tuesday, August 30, 2013

Project title: Identification of Staphylococcal Immune Modulatory Ligands Using Transposonomics

Project description: This project will continue on earlier work published by the lab showing that Staphylococcus aureus can downregulate the T cell response to staphylococcal superantigens by inducing IL-10 secretion in a TLR2-dependent mechanism. We hypothesize that S. aureus contains two types of ligands: ones that induce a predominantly a pro-inflammatory response and others that induce an anti-inflammatory response. Using transposonomics, a novel high throughput technique we have developed, we have identified a panel of mutants with enhanced or reduced IL-10-inducing capacity, compared to the parental strain. The project for this student will focus on characterizing the immuno-modulatory profile of several of these mutants. The student will become familiar with basic microbiology techniques, isolating and stimulating human PBMCs, standard tissue culture techniques, and functional ELISA-based assays.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

How students can apply: For MIMM: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Human subjects; Biohazardous substances; Handling chemicals.