'Initation of polyomavirus DNA replication' - MIMM 396 Undergraduate Research Project Application Form

Supervisor's Name: Jacques Archambault

Supervisor's Email: jacques.archambault2 [at] mcgill.ca

Supervisor's Phone: 514-987-5739

Supervisor's Website: http://www.ircm.qc.ca/LARECHERCHE/Axes/Biologie/Virologie/Pages/default....

Supervisor's department: Microbiology and Immunology

Course number: MIMM 396 (Microbiology)

Term: Summer 2013

Project start date: Monday, June 3, 2013

Project end date: Tuesday, April 16, 2013

Project title: Initation of polyomavirus DNA replication

Project description: In this project the student will investigate the mechanims by which the large T antigen (LTA) helicase of simian virus 40 (SV40) assembles at the viral origin of DNA replication in vivo. This project will make use of cellular, luciferase-based, SV40 DNA replication assay (Fradet-Turcotte et al., 2010, Virology, 399:65-76). Mutations will be introduced in the origin of repication to test the requirment of specfic DNA elements. Similalry, LTA mutant proteins will be created. The ability of these mutant origins and LTA proteins to support viral DNA replication will then be tested in transfected cells.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% research report paper; 50% laboratory research performance.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves biohazardous substances.