Real time measurement of airway surface liquid composition of respiratory epithelial cells - PHGY 396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may simply print this webpage. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).

QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.

Supervisor's Name: Gergely Lukacs

Supervisor's Email: gergely.lukacs [at]

Supervisor's Phone: 514-398-5582

Supervisor's Website:

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Real time measurement of airway surface liquid composition of respiratory epithelial cells

Project description (50-100 words suggested): Impaired functional expression of CFTR alters the composition of the airway surface liquid, a phenomenon implicated in the pathogenesis of cystic fibrosis. We plan to set up model to compare the pH, chloride and glutathione concentrations in real-time, using wild type and delta508 CFTR expressing polarized human respiratory epithelial cell on filter support under physiological conditions.  This will be accomplished by engineering various fluorescence protein sensors (ClopHensor and Grx1-roGFP2) that are specifically targeted to the extracellular surface of the apical plasma membrane. Epithelial cells will be transduced by lentivirus and the fluorescence will be monitored by laser confocal fluorescence microscopy.  The effect of various metabolic conditions and CFTR channel activation will also be examined.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (50%) and a final written report (50%)  by the supervisor.

Project status: This project is taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances.

Student's Name:

Student's McGill ID:

Student's McGill Email:

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I certify that this course is with a different supervisor and on a different topic than any previous '396' course I have taken. I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:


Unit chair/director/designate's name:

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses: