Serotonergic neuron activation by hypoxia - NSCI 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Maria Pompeiano
Supervisor's Email: maria [dot] pompeiano [at] mcgill [dot] ca
Supervisor's Phone: 514-398 6080
Supervisor's department: Psychology
Course number: NSCI 396 (Neuroscience)
Term: Winter 2013-2014
Project start date: Monday, January 6, 2014
Project end date: Friday, April 11, 2014
Project title: Serotonergic neuron activation by hypoxia
Project description (50-100 words suggested): In mammals, the serotonergic neurons of the medullary Raphe are vital for temperature homeostasis and participate in the hypothermic, hypometabolic, and cardiorespiratory responses to hypoxia. In adult rats, hypoxia induces 5HT neuron activation (revealed by cFos immunoreactivity) in the raphe magnus (B3), raphe pallidus (B1), and nearby parapyramidal neurons, particularly in the more rostral areas, but not in the raphe obscurus (B2). The present project aims at studying a possible activation of regulatory serotonergic neurons in embryos exposed to hypoxia, by using double-labelling fluorescent immunohistochemistry with antibodies against tryptophan hydroxylase (marker for serotonergic neurons) and cFos (a marker of neuronal activity). The physiological responses to hypoxia are similar in adults and embryos, with an important difference in the respiratory responses. In fact, adults increase respiratory movement while embryos decreases them following hypoxia exposure. We will test the hypothesis that serotonergic neurons of the raphe pallidus will be activated by hypoxia (as seen by an increased cFos expression) as compared to normoxia controls while the raphe magnus will show the opposite reaction. In fact, the raphe magnus appears to be responsible for the hypoxic ventilatory response in adults.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory performance (50%) and a final written report (50%).
Project status: This project is taken.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves handling chemicals.