Genomic imprinting during meiosis - HGEN 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Anna Naumova
Supervisor's Email: anna [dot] naoumova [at] mcgill [dot] ca
Supervisor's Phone: 514-934-1934 x 35906
Supervisor's department: Human Genetics
Course number: HGEN 396 (Human Genetics)
Term: Winter 2013-2014
Project start date: Monday, January 6, 2014
Project end date: Friday, April 11, 2014
Project title: Genomic imprinting during meiosis
Project description (50-100 words suggested): Genomic imprinting is an epigenetic mechanism that causes monoallelic parent-of-origin dependent gene expression in mammals. Genomic imprints are reset during gametogenesis to ensure normal development of the future mammalian embryos. However, little is known about the exact timing of imprint resetting and transcriptional regulation of imprinted genes during meiosis. Several studies suggest that imprint resetting is complete before the onset of meiosis. Our preliminary data suggest that completion of resetting does not occur until the onset of the first meiotic division. The goal of this project is to refine the timing of imprint establishment during spermatogenesis. Completion of imprint establishment is expected to cause biallelic expression of imprinted genes. The student will analyze allelic expression of four imprinted genes in mouse testis to determine the stage when their expression becomes biallelic (e.g. genes are expressed from both chromosomes). This work will require conducting RNA extraction, reverse transcription and PCR, running agarose gels and analyzing the results.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor. Details of the evaluation scheme will be provided by HGEN to supervisors and applicants.
Other project information: The student should have a good knowledge of basic chemistry, genetics, and molecular biology, manual dexterity and enthusiasm for research. The project will require mouse handling.
Project status: This project is taken. The professor has no more '396' projects this term.
How students can apply / Next steps: For HGEN: After all of the parts of this application forms are completed and the hard copy is signed by the professor and the student, bring the application form and a copy of your unofficial transcript to the Department of Human Genetics (to the attention of Dr. Patricia N. Tonin, Stewart Biology Building N5.13) during office hours.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects.