Cell-in-alginate trap-array with sequential antibody delivery for multiplex immunoassays on single-cells - CHEM 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Amy S. Blum
Supervisor's Email: amy [dot] blum [at] mcgill [dot] ca
Supervisor's Phone: 514-398-6237
Supervisor's department: Chemistry
Course number: CHEM 396 (Chemistry)
Term: Winter 2013-2014
Project start date: Monday, January 6, 2014
Project end date: Friday, April 11, 2014
Project title: Cell-in-alginate trap-array with sequential antibody delivery for multiplex immunoassays on single-cells
Project description (50-100 words suggested): In this project we would like to design and implement a trap-array for cells which may be used for multiplex immunoassays on live, isolated CTCs. The platform could allow the CTC team to find proteomic and structural differences from captured vs non-captured spiked living CTCs, and possibly find new biomarkers which could help their capture. The basic premise being: CTCs are co-encapsulated with capture-beads in an alginate-in-oil droplet and arrayed on a microfluidic chip similar to the designed developed by Tan et al. . Then, labeled-dAbs are delivered sequentially to the cells. Using only one fluorophore, multiplex immunoassays may be performed on live cells based on time-discrimination.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): 50% final report, 25% lab grade, 25% oral presentation
Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.