'The effect of pCO2 on activation of Src/Pyk2 and NKCC' - PHGY 396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may simply print this webpage. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).

QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.

Supervisor's Name: John W. Hanrahan

Supervisor's Email: John [dot] hanrahan [at] mcgill [dot] ca

Supervisor's Phone: Tel. (514) 398-8320

Supervisor's Website:

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: The effect of pCO2 on activation of Src/Pyk2 and NKCC

Project description (50-100 words suggested): pCO2 at the apical surface of airway epithelial cells varies from 0.035% (when room air is inhaled) to 5% (when alveolar air is exhaled). The impact of these large pCO2 oscillations on intracellular pH and bicarbonate transport have not been studied. Src and Pyk-2 sense intracellular acidification in kidney cells and activate sodium bicarbonate cotransporters (Espiritu, Bernardo et al. 2002) and sodium proton exchangers (Tsuganezawa, Preisig et al. 1998). The student will study the effect of acute changes in pCO2 on airway epithelial cells and non-epithelial cells. Air-liquid interface cultures will be moved from an incubator containing room air to one containing 5% pCO2 for 5 min. Activation of Src and Pyk-2 will be monitored by probing Western blots cell lysates with phospho-specific antibodies.  If they are activated, the student will test if NBCe1 and H+ transporters also become phosphorylated on tyrosine by stripping and reprobing the blots with transporter- and phosphotyrosine-specific antibodies. These studies should provide new insight into acid-base regulation in airway epithelial cells.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade):

Project status: This project is taken.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances; Handling chemicals.

Student's Name:

Student's McGill ID:

Student's McGill Email:

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I certify that this course is with a different supervisor and on a different topic than any previous '396' course I have taken. I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:


Unit chair/director/designate's name:

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses: