'Photoconversion of Nuclear Probes' - PHGY 396 Undergraduate Research Project Application Form
INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").
INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.
INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).
QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.
Supervisor's Name: Claire Brown
Supervisor's Email: claire [dot] brown [at] mcgill [dot] ca
Supervisor's Phone: 541-677-7493
Supervisor's Website: http://www.mcgill.ca/physiology/directory/core-faculty/claire-brown/
Supervisor's department: Physiology
Course number: PHGY 396 (Physiology)
Term: Fall 2013-2014
Project start date: Tuesday, September 3, 2013
Project end date: Tuesday, December 3, 2013
Project title: Photoconversion of Nuclear Probes
Project description (50-100 words suggested): Fluorescent nuclear probes have been shown to convert from fluorescent blue to fluorescent green upon excitation with UV light and 405 nm laser light. This photoconversion can lead to artifacts in images containing green fluorescent labels leading to overestimates of green intensities and false positives for nuclear localization of green fluoescent probes. This project will involve testing different UV and 405 nm excitation powers to determine if there is a critical time or intensity threshold important for this photoconversion from blue to green to occur. The end result will be a set of recommendations for scientists using these blue nuclear dyes so they can ensure they do not induce this photobleaching artifact during standard fluorescence imaging.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): 50% for the written report and 50% for the laboratory work.
Project status: This project is taken. The professor has no more '396' projects this term.
How students can apply: Contact me by email and send me a copy of your transcript (unofficial copy is fine).
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.