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'Photobleaching rates and pulsed light' - PHGY 396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).

QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.


Supervisor's Name: Claire Brown

Supervisor's Email: claire [dot] brown [at] mcgill [dot] ca

Supervisor's Phone: 541-677-7493

Supervisor's Website: http://www.mcgill.ca/physiology/directory/core-faculty/claire-brown

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Photobleaching rates and pulsed light

Project description (50-100 words suggested): It has recently been shown that using pulsed laser light reduced photobleaching and phototoxicity in fluorescence microscopy and live cell imaging. This has the benefit of preserving fluorescence during light intensive confocal laser scanning microscope (CLSM) imaging. It also has the benefit of preserving living samples during live cell timelapse imaging. This project will test the dependence of photobleaching of fixed enhanced green fluorescent protein (EGFP) labelled cells with different scanning configurations on the CLSM. The student will learn how to use the confocal microscope, collect images and perform image processing and analysis to extract bleach rates.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% for the laboratory work and 50% for the written report.

Project status: This project is taken.

How students can apply: Contact me by email and send me a copy of your transcript (unofficial copy is fine)

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: None of the above (NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers).

Student's Name:


Student's McGill ID:


Student's McGill Email:


Student's Phone:


Student's Program:


Student's Level (U0 / U1 / U2 / U3):


Student's signature - I certify that this course is with a different supervisor and on a different topic than any previous '396' course I have taken. I have not applied for another '396' course in this term:


Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:


Date:


Unit chair/director/designate's name:


Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses:


Date: