'Initation of polyomavirus DNA replication' - MIMM 396 Undergraduate Research Project Application Form

INSTRUCTIONS - PROFESSORS: Please print and review this form. Complete or correct the sections, as applicable, from "Supervisor's Name" to "Ethics, safety, and training". Please sign and date near the bottom ("Supervisor's signature").

INSTRUCTIONS - STUDENTS: You may receive this form by email, or you may download it after it has been posted here. Either way, print and review this form. Complete or correct the sections, from "Student's Name" to "Student's Level", and sign ("Student signature"). Ask your supervisor to sign her/his section near the bottom. Take it to the department* corresponding to the course number in Section A; this may or may not be your own department. (* EXCEPTIONS: For NSCI 396 and COGS 396, please bring it to the Interdisciplinary Programs Adviser in Dawson Hall.) Do not register for a '396' course on Minerva until you receive departmental permission. Have a discussion with your supervisor about time/work expectations, keeping in mind that this is a 3-credit course (roughly, 10 hours per week for 12 weeks). Remember that a '396' course is an elective.

INSTRUCTIONS - DEPARTMENTS: After the unit chair/director/designate approves (or not) this project, please notify student. If approved, please give student permission to register on Minerva, and send a copy of this form (with signatures) to the Office for Undergraduate Research in Science (either fax, or internal mail to Dawson Hall 408-A, or PDF scan + email).

QUESTIONS OR FEEDBACK? Contact the Office for Undergraduate Research in Science.

Supervisor's Name: Jacques Archambault

Supervisor's Email: jacques [dot] archambault2 [at] mcgill [dot] ca

Supervisor's Phone: 514-987-5739

Supervisor's Website: http://www.ircm.qc.ca/LARECHERCHE/Axes/Biologie/Virologie/Pages/default....

Supervisor's department: Microbiology and Immunology

Course number: MIMM 396 (Microbiology)

Term: Summer 2013

Project start date: Monday, June 3, 2013

Project end date: Tuesday, April 16, 2013

Project title: Initation of polyomavirus DNA replication

Project description: In this project the student will investigate the mechanims by which the large T antigen (LTA) helicase of simian virus 40 (SV40) assembles at the viral origin of DNA replication in vivo. This project will make use of cellular, luciferase-based, SV40 DNA replication assay (Fradet-Turcotte et al., 2010, Virology, 399:65-76). Mutations will be introduced in the origin of repication to test the requirment of specfic DNA elements. Similalry, LTA mutant proteins will be created. The ability of these mutant origins and LTA proteins to support viral DNA replication will then be tested in transfected cells.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% research report paper; 50% laboratory research performance.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves biohazardous substances.

Student's Name:

Student's McGill ID:

Student's McGill Email:

Student's Phone:

Student's Program:

Student's Level (U0 / U1 / U2 / U3):

Student's signature - I certify that this course is with a different supervisor and on a different topic than any previous '396' course I have taken. I have not applied for another '396' course in this term:

Supervisor's signature - I give my permission for the student identified above to register for this project under my supervision:


Unit chair/director/designate's name:

Unit chair/director/designate's signature - I certify that this project conforms to departmental requirements for 396 courses: